Fig. 4From: Droplet digital polymerase chain reaction (ddPCR) for the detection of Plasmodium knowlesi and Plasmodium vivaxStandard curve of qPCR assay run with positive plasmid DNA of Plasmodium knowlesi Plasmepsin. Plasmid DNA was ten-fold diluted serially from 1000 to 0.01 copies/μL. The slope of the plasmid DNA standard curve is − 3.893, equivalent to an efficiency of 80.7% (R2 = 0.799)Back to article page