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Table 2 Analysis of clinical samples using ddPCR compared to nested PCR

From: Droplet digital polymerase chain reaction (ddPCR) for the detection of Plasmodium knowlesi and Plasmodium vivax

Sample

ddPCR (number of samples)

Number of samples

Positive

Negative

P. vivax

P. knowlesi

Mixed (P. vivax and P. knowlesi)

Nested PCR-confirmed P. vivax

28

0

4

8

40

Nested PCR-confirmed P. knowlesi

0

28

8

4

40

P. malariae

0

0

0

1

1

P. falciparum

0

0

0

1

1

P. ovale

0

0

0

1

1

T. gondii

0

0

0

1

1

D. immitis

0

0

0

1

1

Healthy donors

0

9

0

8

17

Microscopy-negative

1

1

0

10

12