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Table 3 Comparison of results between nested PCR, ddPCR and qPCR

From: Droplet digital polymerase chain reaction (ddPCR) for the detection of Plasmodium knowlesi and Plasmodium vivax

Samples

Total

ddPCR result

qPCR result

Pv

Pk

Pv + Pk

Negative

Pv

Pk

Pv + Pk

Negative

Nested PCR-confirmed Pv only

30

24

0

2

4

23

0

6

1

Nested PCR-confirmed Pk only

29

0

21

5

3

1

21

7

0

Other Plasmodium spp.

2

0

0

0

2

0

0

0

2

Microscopy - negative

12

1

1

0

10

3

0

0

9

Total

73

25

22

7

19

27

21

13

12

  1. Pv, P. vivax; Pk, P. knowlesi