Table 4 Number and type of samples in agreement or discordance based on results from ddPCR and qPCR assays

Nested PCR-confirmed Pv only (n = 30)

19

–

–

1

Nested PCR-confirmed Pk only (n = 29)

–

15

1

–

Other Plasmodium spp. (n = 2)

–

–

–

2

Microscopy–negative (n = 12)

1

–

–

9

Discordance

qPCR result – ddPCR result

Pv (qPCR) − Pv + Pk (ddPCR)

Pv + Pk (qPCR) − Pv (ddPCR)

Pv (qPCR) - negative (ddPCR)

Pv + Pk (qPCR) − negative (ddPCR)

Nested PCR-confirmed Pv only (n = 30)

2

5

2

1

Pk (qPCR) − Pv + Pk (ddPCR)

Pv (qPCR) − Pv + Pk (ddPCR)

Pv + Pk (qPCR) − Pk (ddPCR)

Pk (qPCR) − negative (ddPCR)

Nested PCR-confirmed Pk only (n = 29)

3

1

6

3

Pv (qPCR) − Pk (ddPCR)

Pv (qPCR) − negative (ddPCR)

Microscopy-negative (n = 12)

1

1