Fig. 2
Tethered female Anopheles gambiae s.l.a Entomological pin attached to ventral side of posterior abdomen of the mosquito (a; right), allowing unobstructed flight (a; left). Bottom part of pin inserted into clipped 10 µl pipet tip as a base. b Tethered mosquitoes in recuperation time before assay start, fore legs resting on folded paper for tarsal contact preventing flight. c Tether flight hive of 18 flight tubes housed inside soft (mattress) foam for surrounding sound muffling and external cue reduction. Each flight tube microphone connects to an individual sound recorder. ci Tethered female inside flight tube (polystyrene prototype, not used in the experiment); tethered mosquito construct attached on to double-sided foam tape with microphone (black) in backdrop. Photos by: RF (a and ci) and ASY (b and c)