Use of a NAT-based assay to improve the surveillance system and prevent transfusion-transmitted malaria in blood banks

Table 2 Cycle threshold (Ct) results for FMT–HVD samples using the BioRobot MDx (silica extraction) and NAT Plus System (bead extraction) workstations, and in-house 18S RNA gene quantification

Patient	Sample	MDx (silica)		NAT Plus (beads)		18S RNA quantification
Patient	Sample	Single (Ct value)	Pool of six (Ct value)	Single (Ct value)	Pool of six (Ct value)	Copies/µL
1	Whole blood	12.45	14.36			550,345.9
1	Plasma	16.1	17.53	15.64	18.51	22,732.9
2	Whole blood	18.66	20.22			38,766.9
2	Plasma	17.53	20.33	21.41	19.91	89.5
3	Whole blood	20.47	23.61			13,727.3
3	Plasma	25.99	28.83	20.01	24.96	150.8
4	Whole blood	13.17	15.17			53,443.5
4	Plasma	23.42	27.4	19.58	22.95	161.7
5	Whole blood	17.19	21.87			194,645.8
5	Plasma	20.95	24.23	17.45	21.08	399.9
6	Whole blood	15.7	21.29			103,623.2
6	Plasma	20.16	26.7	15.39	19.74	192.9
7	Whole blood	18.2	20.08			368,428.4
7	Plasma	22.32	25.61	19.75	23.38	24,047.6
8	Whole blood	25.22	29.92			161.5
8	Plasma	Non-reagent	Non-reagent	28.12	31.84	7.1
9	Whole blood	18.91	21.97			14,431.3
9	Plasma	23.61	26.75	20.51	24.09	1155.3
10	Whole blood	24.43	26.93			1358.9
10	Plasma	28.76	Non-reagent	26.07	28.49	2.9
11	Whole blood	12.35	14.71			186,777.7
11	Plasma	26.53	29.1	22.52	25.74	471.7
12	Whole blood	11.89	14.0			239,674.9
12	Plasma	26.7	29.36	22.67	25.9	109.2
13	Whole blood	13.37	15.71			372,567.5
13	Plasma	22.57	25.73	18.3	21.44	1156.3
14	Whole blood	16.86	19.5			19,880.9
14	Plasma	27.23	29.8	21.84	25.84	113.2
15	Whole blood	12.53	14.96			310,122.1
15	Plasma	24.38	27.1	19.79	23.64	137.9
16	Whole blood	12.94	15.39			102,256.4
16	Plasma	21.48	23.74	16.23	19.86	739.8

ISSN: 1475-2875