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Table 2 Cycle threshold (Ct) results for FMT–HVD samples using the BioRobot MDx (silica extraction) and NAT Plus System (bead extraction) workstations, and in-house 18S RNA gene quantification

From: Use of a NAT-based assay to improve the surveillance system and prevent transfusion-transmitted malaria in blood banks

Patient Sample MDx (silica) NAT Plus (beads) 18S RNA quantification
Single (Ct value) Pool of six (Ct value) Single (Ct value) Pool of six (Ct value) Copies/µL
1 Whole blood 12.45 14.36    550,345.9
Plasma 16.1 17.53 15.64 18.51 22,732.9
2 Whole blood 18.66 20.22    38,766.9
Plasma 17.53 20.33 21.41 19.91 89.5
3 Whole blood 20.47 23.61    13,727.3
Plasma 25.99 28.83 20.01 24.96 150.8
4 Whole blood 13.17 15.17    53,443.5
Plasma 23.42 27.4 19.58 22.95 161.7
5 Whole blood 17.19 21.87    194,645.8
Plasma 20.95 24.23 17.45 21.08 399.9
6 Whole blood 15.7 21.29    103,623.2
Plasma 20.16 26.7 15.39 19.74 192.9
7 Whole blood 18.2 20.08    368,428.4
Plasma 22.32 25.61 19.75 23.38 24,047.6
8 Whole blood 25.22 29.92    161.5
Plasma Non-reagent Non-reagent 28.12 31.84 7.1
9 Whole blood 18.91 21.97    14,431.3
Plasma 23.61 26.75 20.51 24.09 1155.3
10 Whole blood 24.43 26.93    1358.9
Plasma 28.76 Non-reagent 26.07 28.49 2.9
11 Whole blood 12.35 14.71    186,777.7
Plasma 26.53 29.1 22.52 25.74 471.7
12 Whole blood 11.89 14.0    239,674.9
Plasma 26.7 29.36 22.67 25.9 109.2
13 Whole blood 13.37 15.71    372,567.5
Plasma 22.57 25.73 18.3 21.44 1156.3
14 Whole blood 16.86 19.5    19,880.9
Plasma 27.23 29.8 21.84 25.84 113.2
15 Whole blood 12.53 14.96    310,122.1
Plasma 24.38 27.1 19.79 23.64 137.9
16 Whole blood 12.94 15.39    102,256.4
Plasma 21.48 23.74 16.23 19.86 739.8