Towards harmonization of microscopy methods for malaria clinical research studies

Malaria Journal

Table 2 Variations in counting parameters with differences in the Field Number (FN) of the ocular lens

Field number	18	20	22	26.5
Area of HPF (mm²)	0.0255	0.0314	0.0380	0.0552
Volume of blood per HPF (µL)	0.00135	0.00167	0.00202	0.00293
Volume of blood per 200 HPFs (µL)	0.270	0.333	0.403	0.585
Mean WBC/HPF (assuming 8000 WBC/µL)	11	13	16	23

Values calculated assuming a standard thick film made with 6 µL of blood evenly spread over a circle of 12 mm diameter [11]

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