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Table 2 Variations in counting parameters with differences in the Field Number (FN) of the ocular lens

From: Towards harmonization of microscopy methods for malaria clinical research studies

Field number 18 20 22 26.5
Area of HPF (mm2) 0.0255 0.0314 0.0380 0.0552
Volume of blood per HPF (µL) 0.00135 0.00167 0.00202 0.00293
Volume of blood per 200 HPFs (µL) 0.270 0.333 0.403 0.585
Mean WBC/HPF (assuming 8000 WBC/µL) 11 13 16 23
  1. Values calculated assuming a standard thick film made with 6 µL of blood evenly spread over a circle of 12 mm diameter [11]