Fig. 3
From: Molecular characterization of Plasmodium falciparum DNA-3-methyladenine glycosylase
Amplification of PfMAG and expression of recombinant PfMAG. a PCR amplification of P. falciparum DNA-3-methyladenine glycosylase gene. Lane M, DNA size ladder; lane 1, PfMAG amplicon. b SDS-PAGE and c western blotting of recombinant PfMAG heterologously expressed in E. coli LMG 194 at 15 °C for 16 h. Recombinant protein was purified using HisTrap HP column and eluted with a 10–500 mM imidazole linear gradient. Lane M, standard protein size markers; lane 1, purified PfMAG eluted at 500 mM imidazole