Fig. 6
From: Molecular characterization of Plasmodium falciparum DNA-3-methyladenine glycosylase
Effects of different buffer conditions on glycosylase activity of recombinant PfMAG. a Assay reaction was carried out under standard conditions except buffer used was 50 mM citrate-sodium citrate pH 3-5, phosphate-citrate pH 6-7 and Tris–Cl pH 7–9. b Effects of NaCl on glycosylase activity of recombinant PfMAG. Assay reaction was carried out under standard conditions but with 0–500 mM NaCl. c Effects of divalent cation on PfMAG activity. The 27-mer double-stranded DNA of εA–T was used as a substrate, and negative control contained no enzyme (− ve)