Fig. 4
Limits of detection for 3D7 and Dd2 P. falciparum DNA by template copy number. The LoDs of qPCR assays for drug-susceptible (3D7) and –resistant (Dd2) P. falciparum DNA decreased (analytical sensitivities increased) as the template copy numbers of the qPCR assays increased (p < 0.001). Note that the TARE-2 assay (n = 160) yielded consistently positive results for the lowest concentration of Dd2 DNA tested initially (10−4 pg DNA per qPCR) but did not yield consistently positive results for an additional dilution of Dd2 DNA (10−5 pg DNA per qPCR)