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Table 1 Template copy number estimates for 13 candidate qPCR assays

From: Template copy number and the sensitivity of quantitative PCR for Plasmodium falciparum in asymptomatic individuals

qPCR assay

References

P. falciparum DNA sequence

Template copy No.a

crt

This article

Chloroquine-resistance transporter

1

crt76 K

[13]

Chloroquine-resistance transporter (76K)

1

crt76T

[13]

Chloroquine-resistance transporter (76T)

1

ldh(a)

[14]

Lactate dehydrogenase

1

ldh(b)

This article

Lactate dehydrogenase

1

18SrRNA(a)

[15]

18S Ribosomal ribonucleic acid

3

18SrRNA(b)

[16]

18S Ribosomal ribonucleic acid

3

r364(a)

[17]

Repetitive element 364

14

cytb

[18]

Cytochrome b

22

coxI

This article

Cytochrome oxidase I

22

varATS

[19]

var gene acidic terminal sequence

29

r364(b)

This article

Repetitive element 364

56

TARE-2

[19]

Telomere-associated repetitive element 2

160

  1. a Template copy number estimates for the P. falciparum DNA sequences yielding positive fluorescent signals (based on the 3D7 P. falciparum genome)