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Fig. 1 | Malaria Journal

Fig. 1

From: Particle-based, Pfs230 and Pfs25 immunization is effective, but not improved by duplexing at fixed total antigen dose

Fig. 1

Duplexed particle formation of Pfs25 and Pfs230C1 with CoPoP/MPLA liposomes. a Schematic representation of the duplexing concept with CoPoP liposomes. b Binding of individual or duplexed antigens to CoPoP/MPLA liposomes following 3 h incubation, using a microcentrifugal filtration assay. c Cryo EM of CoPoP/MPLA liposomes before (left) and after binding duplexed Pfs25 and Pfs230 (right). d Particle size before and after antigen binding. e Native PAGE confirming individual and duplexed antigen binding to CoPoP/MPLA liposomes. The absence of a band in the CoPoP lane is because the antigens attached to the liposomes are too large to enter the gel. Note that Pfs230C1 runs faster than Pfs25 in native PAGE conditions. f SDS-PAGE showing whether the duplex antigens bind to Ni–NTA beads (“B”) or remain in the supernatant (“S”). The antigens bound to CoPoP liposomes remain in the supernatant since the his-tag stably anchors them into the bilayer without capacity to get captured by the beads. Bar graphs in b and d show mean ± std. dev. for n = 3 triplicate experiments

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