Fig. 2

Pfs25 and Pfs230C1 binding to CoPoP/MPLA liposomes characterized using a FRET assay. a Schematic representation of fluorophore-labelled Pfs25 and Pfs230C1 which are quenched after binding to CoPoP/MPLA liposomes, b fluorescence emission spectra of fluorophore-labelled Pfs25 (blue) and Pfs230C1 (red). Based on fluorescence quenching, binding kinetics of the duplexed antigens was assessed in real-time with c CoPoP/MPLA liposomes and d PoP/MPLA liposomes, which lack cobalt in the bilayer. e Serum stability of the duplexed antigens with CoPoP/MPLA liposomes during incubation for 2 weeks in 20% human serum at 37 °C. Data in c, d, and e show mean ± std. dev. for n = 3 triplicate experiments