Fig. 3

Specificity of antibodies induced by mice immunized with duplexed or individual antigens with CoPoP/MPLA liposomes. CD-1 mice were immunized intramuscularly with Pfs25, Pfs230 or the bivalent combination, admixed with CoPoP/MPLA liposomes on day 0 and day 21. Sera were collected on day 42. a Anti-Pfs25 and b Anti Pfs230C1 IgG titres were assessed. ELISA experiments were performed with n = 10 mice and the line represents the geometric mean. One-way ANOVA followed by Tukey test, using log transformed data was used to compared difference^{. **}p = 0.0058, ^***p < 0.0001. c Indirect immunofluorescence assay of NF54 parasites at gametocyte and ookinete stages, reacted with indicated post-immune mouse sera. Images were taken from a single experiment