Fig. 1
From: Human plasma plasminogen internalization route in Plasmodium falciparum-infected erythrocytes
Detection of human plasminogen in cellular fractions from uninfected and infected erythrocytes by Western blotting. The uninfected erythrocytes were lysed by hypotonic solution, and the plasma membrane fraction was separated from the cytosol by centrifugation. Trophozoite-infected erythrocytes were lysed with saponin, and the supernatant containing the cytosol and plasma membrane of infected erythrocytes was separated by 100,000g ultracentrifugation. The parasite pellet was lysed by hypotonic solution and subjected to ultracentrifugation to eliminate membrane fractions and obtain only the parasite cytoplasm fraction