Molecular epidemiological surveillance of Africa and Asia imported malaria in Wuhan, Central China: comparison of diagnostic tools during 2011–2018

Table 1 Primers and probes for Plasmodium species detection

Method	Species	Primer	Sequence (5′–3′)	Length (bp)	References
Nested PCR	Plasmodium sp.	rPLU1	TCAAAGATTAAGCCATGCAAGTGA	1670	[43]
	Plasmodium sp.	rPLU5	CCTGTTGTTGCCTTAAACTTC	1670
	P. falciparum	rFAL1	TTAAACTGGTTTGGGAAAACCAAATATATT	205
	P. falciparum	rFAL2	ACACAATGAACTCAATCATGACTACCCGTC	205
	P. vivax	rPVIV1	CGCTTCTAGCTTAATCCACATAACTGATAC	121
	P. vivax	rPVIV2	ACTTCCAAGCCGAAGCAAAGAAAGTCCTTA	121
	P. malariae	rMAL1	ATAACATAGTTGTACGTTAAGAATAACCGC	145
	P. malariae	rMAL2	AAAATTCCCATGCATAAAAAATTATACAAA	145
	P. ovale	rOVA1WC	TGTAGTATTCAAACGCAGT	659–662
	P. ovale	rOVA2WC	TATGTACTTGTTAAGCCTTT	659–662
	P. ovale curtisi	rOVA1	ATCTCTTTTGCTATTTTTTAGTATTGGAGA	800	[44]
	P. ovale curtisi	rOVA2	GGAAAAGGACACATTAATTGTATCCTAGTG	800
	P. ovale wallikeri	rOVA1v	ATCTCCTTTACTTTTTGTACTGGAGA	780
	P. ovale wallikeri	rOVA2v	GGAAAAGGACACTATAATGTATCCTAATA	780
Real-time PCR	Plasmodium sp.	Plasmo 1	GTTAAGGGAGTGAAGACGATCAGA		[35]
	Plasmodium sp.	Plasmo 2	AACCCAAAGACTTTGATTTCTCATAA
	P. falciparum	P. fal-probe	FAM-AGCAATCTAAAAGTCACCTCGAAAGATGACT-TAMRA
	P. vivax	P. v-probe	HEX-AGCAATCTAAGAATAAACTCCGAAGAGAAAATTCT-TAMRA
	P. malariae	P. m-probe	FAM-CTATCTAAAAGAAACACTCAT-MGB
	P. ovale	P. o-probe	HEX-CGAAAGGAATTTTCTTATT-MGB
	P. ovale	POF	ATAAACTATGCCGACTAGGTT		[42]
	P. ovale	POR	ACTTTGATTTCTCATAAGGTACT
	P. ovale curtisi	POC-probe	FAM-TTCCTTTCGGGGAAATTTCTTAGA-BHQ1
	P. ovale wallikeri	POW-probe	HEX-AATTCCTTTTGGAAATTTCTTAGATTG-BHQ1