Molecular epidemiological surveillance of Africa and Asia imported malaria in Wuhan, Central China: comparison of diagnostic tools during 2011–2018

Table 2 Determination of Plasmodium species by microscopy examination and molecular diagnosis

Microcopic	Parasitaemia (No. of parasites/μl)			Nested PCR^a		Real-time PCR
Microcopic	Species	Intensity (parasite/ul)	No.	Species	No.	Species	No.
Microcopy positive	P. falciparum	Very Low (≤ 100)	7	Pf	6	Pf	5
		Low (101–500)	26	Pf	25	Pf	21
		Low-middle (501–3000)	33	Pf	32	Pf	32
		Middle (3001–10,000)	30	Pf	30	Pf	30
		Middle-high (10,001–100,000)	96	Pf	96	Pf	96
		High (> 100,000)	50	Pf	50	Pf	50
		Error correction (3000)	1	Pf + Po	1	Pf + Po	1
	P. vivax	From 100 to 10,000	15	Pv	15	Pv	15
	P. vivax	Error correction	2	Pv + Po	2	Pv + Po	2
	P. ovale	From 100 to 10,000	19	Po	19	Po	21
	P. ovale	Error correction	2	Pf^a +Po	2	Pf +Po	0
	P. malariae	From 100 to 10,000	6	Pm	6	Pm	6
	P. falciparum + P. ovale	50,000	1	Po	1	Po	1
Microcopy negative	P. falciparum^b		7	Pf	5	Pf	3
Microcopy negative	Suspected case		1	Pf	1	Pf	1
Total			296		291		284

^aThe nested PCR products of these cases have been confirmed by DNA sequencing. ^b These cases were RDT positive for Pf

ISSN: 1475-2875