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Table 2 Determination of Plasmodium species by microscopy examination and molecular diagnosis

From: Molecular epidemiological surveillance of Africa and Asia imported malaria in Wuhan, Central China: comparison of diagnostic tools during 2011–2018

Microcopic Parasitaemia (No. of parasites/μl)   Nested PCRa Real-time PCR
Species Intensity (parasite/ul) No. Species No. Species No.
Microcopy positive P. falciparum Very Low (≤ 100) 7 Pf 6 Pf 5
Low (101–500) 26 Pf 25 Pf 21
Low-middle (501–3000) 33 Pf 32 Pf 32
Middle (3001–10,000) 30 Pf 30 Pf 30
Middle-high (10,001–100,000) 96 Pf 96 Pf 96
High (> 100,000) 50 Pf 50 Pf 50
Error correction (3000) 1 Pf + Po 1 Pf + Po 1
P. vivax From 100 to 10,000 15 Pv 15 Pv 15
Error correction 2 Pv + Po 2 Pv + Po 2
P. ovale From 100 to 10,000 19 Po 19 Po 21
Error correction 2 Pfa +Po 2 Pf +Po 0
P. malariae From 100 to 10,000 6 Pm 6 Pm 6
P. falciparum + P. ovale 50,000 1 Po 1 Po 1
Microcopy negative P. falciparumb 7 Pf 5 Pf 3
Suspected case 1 Pf 1 Pf 1
Total 296   291   284
  1. aThe nested PCR products of these cases have been confirmed by DNA sequencing. b These cases were RDT positive for Pf