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Table 1 Plasmodium falciparum pm2 copy number determination with listing of quantitative PCR primers and protocol

From: No evidence of amplified Plasmodium falciparum plasmepsin II gene copy number in an area with artemisinin-resistant malaria along the China–Myanmar border

Primer sequence Sequences Tm (℃) Product size (bp) Range of melt temperature (℃)
Pf pm2_CN_F 5′-TGGTGATGCAGAAGTTGGAG-3′ 59.8 79 76.8–77.2
Pf pm2_CN _R 5′-TGGGACCCATAAATTAGCAGA-3′ 59.4
Pf β-tubulin_CN_F 5′-TGATGTGCGCAAGTGATCC-3′ 61.9 79 79.0–79.2
Pf β-tubulin_CN_R 5′-TCCTTTGTGGACATTCTTCCTC-3′ 60.5