Fig. 4

SERA amplification, M is 100 bp molecular weight marker, 1–7 are field samples, K1, HB3 are laboratory isolates of Plasmodium falciparum used as positive controls, −ve is the negative control. The expected product size is at 175 bp (ORI) and 199 bp (ORII). b EBA-175 amplification, M, 100 bp molecular weight marker, lane 1 and K1-mixed infections with both 795 bp and 714 bp alleles. Lanes 2, 6, 8, 10, Dd2 and 3D7 infection with the F-allele at 795 bp, while lanes 3, 5 and 7 represent the C-allele at 714 bp. −VE and −N are the negative and nested negative controls. c A gel picture of CSP amplification, M, 100 bp molecular weight marker, lanes 1–7 are field isolates, K1 and 3D7 are positive controls, −ve is a negative control. The expected product size is at 321 bp