Community-based surveys for Plasmodium falciparum pfhrp2 and pfhrp3 gene deletions in selected regions of mainland Tanzania

Bakari, Catherine; Jones, Sophie; Subramaniam, Gireesh; Mandara, Celine I.; Chiduo, Mercy G.; Rumisha, Susan; Chacky, Frank; Molteni, Fabrizio; Mandike, Renata; Mkude, Sigsbert; Njau, Ritha; Herman, Camelia; Nace, Douglas P.; Mohamed, Ally; Udhayakumar, Venkatachalam; Kibet, Caleb K.; Nyanjom, Steven G.; Rogier, Eric; Ishengoma, Deus S.

doi:10.1186/s12936-020-03459-3

Table 2 Summary of pfhrp2 and pfhrp3 genotyping results for the samples selected based on field RDT and microscopy results, and laboratory antigen assay or both

From: Community-based surveys for Plasmodium falciparum pfhrp2 and pfhrp3 gene deletions in selected regions of mainland Tanzania

Sample #	Selected on:	Village	Age (yrs)	RDT Results	Microscopy	PET Pf Ct	pAldo^a (ng/mL)	pLDH^a (ng/mL)	HRP2 (ng/mL)	pfhrp2 exons 1/2	pfhrp3 exons 1/2	pf msp1^b	pf msp2^b
Sample #	Selected on:	Village	Age (yrs)	RDT Results	Parasite density (p/µL)	PET Pf Ct	pAldo^a (ng/mL)	pLDH^a (ng/mL)	HRP2 (ng/mL)	pfhrp2 exons 1/2	pfhrp3 exons 1/2	pf msp1^b	pf msp2^b
1	aldo	Chiulu	6	+	256	35.1	43.9	3.7	1.0	+ / +	+ / +
2	aldo	Chiulu	6	−	0	35.6	40.6	2.7	0.0	+ / +	+ / +
3	rdt	Chiulu	33	−	80	38.3	0.0	0.0	0.0	+ / +	+ / +
4	rdt	Herembe	3	−	520	31.8	28.4	2.8	1.0	+ / +	+ / +
5	ldh	Kasenga	54	+	1717	33.8	26.0	6.0	1.0	+ / +	+ / +
6	ldh	Kasenga	10	+	1840	33.5	30.6	10.6	1.9	+ / +	+ / +
7	aldo	Kasenga	6	+	591	35	62.3	6.0	1.7	+ / +	+ / +
8	aldo	Kasenga	4	+	440	33.9	46.5	4.1	1.4	+ / +	+ / +
9	rdt	Kasenga	4	−	32	35	0.0	0.0	0.0	+ / +	+ / +
10	rdt	Kasenga	13	−	96	33.3	0.0	0.0	0.8	+ / +	+ / +
11	rdt	Kasenga	0	−	221	33.7	0.0	0.0	0.0	+ / +	+ / +
12	ldh aldo	Katale	2	+	480	31.8	360.9	114.6	21.2	+ / +	+ / +
13	ldh aldo	Katale	4	+	2628	28.7	797.1	214.5	42.4	+ / +	+ / +
14	ldh aldo	Katale	10	−	0	35.3	76.4	8.9	0.2	+ / +	+ / +
15	ldh aldo rdt	Katale	9	−	2000	28.2	210.8	62.4	5.0	+ / +	+ / +
16	aldo rdt	Katale	4	−	280	31	44.1	0.0	0.0	+ / +	−/−	−/−	+ / −
17	ldh aldo	Kigege	2	+	48	32.4	190.5	80.3	12.4	+ / +	+ / +
18	rdt	Kigege	38	−	16	38.9	0.0	0.0	0.0	+ / +	−/ +	−/ +	−/−
19	rdt	Kigege	8	−	520	34.8	0.0	0.0	0.0	+ / +	+ / +
20	ldh	Kitunguli	6	+	0	31.5	42.9	39.2	1.5	+ / +	+ / +
21	rdt	Mkowela	43	-	440	37.3	0.0	0.0	0.0	+ / +	±	+ / −	−/ +
22	aldo	Mkowela	2	+	0	35.3	51.0	5.4	1.3	+ / +	+ / +
23	ldh aldo	Mkowela	11	−	0	35.8	46.3	5.6	0.0	+ / +	+ / +
24	aldo	Mkunwa	3	+	48	36	33.0	5.9	0.1	+ / +	+ / +
25	rdt	Mtawarawa	14	−	176	33.2	0.0	0.0	0.0	+ / +	+ / +
26	rdt	Mtawarawa	22	−	96	34.9	0.0	0.0	0.1	+ / +	+ / +
27	ldh	Mwamila	2	+	11,480	27.6	338.3	159.4	35.5	+ / +	+ / +
28	ldh aldo rdt	Nyangalamila	8	−	288	34	54.7	11.0	0.0	+ / +	+ / +
29	ldh aldo	Nyankoronko	7	+	104,000	25.7	760.1	242.9	37.5	+ / +	+ / +
30	ldh aldo	Nyankoronko	2	+	160	33.8	98.8	18.3	2.1	+ / +	+ / +
31	ldh aldo	Nyankoronko	4	+	28,800	29.8	76.2	19.1	0.9	+ / +	+ / +
32	ldh	Nyankoronko	6	+	440	36.5	24.2	7.9	1.4	+ / −	+ / +	+ / −	+ / −
33	aldo rdt	Nyankoronko	43	−	48	37	31.3	0.0	0.0	+ / +	+ / +
34	rdt	Nyankoronko	10	−	760	31.3	0.0	0.0	0.8	+ / +	+ / +
35	ldh rdt	Nyankoronko	9	−	88,600	25.5	187.5	85.1	16.6	+ / +	+ / +

^aAntigens listed indicate aberrant relationship between HRP2 and that antigen’s assay signal; RDT indicates microscopy/RDT discordance
^bGenotyping for pfmsp1 and pfmsp2 not performed if the sample showed amplification of all pfhrp2 and pfhrp3 targets

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