Table 1 Primer sequences used for the nested PCR and restriction enzymes used for the RLFP
Primer name | Sequence | Restriction enzyme |
|---|---|---|
Nested1 forward Pfmdr1 86 (MDR-A1) | TGTTGAAAGATGGGTAAAGAGCAGAAAGAG | AflIII (NEB) |
Nested1 reverse Pfmdr1 86 (MDR-A3) | TACTTTCTTATTACATATGACACCACAAACA | |
Nested2 forward Pfmdr1 86 (MDR-A4) | AAAGATGGTAACCTCAGTATCAAAGAAGAG | |
Nested2 reverse Pfmdr1 86 (MDR-A2) | GTCAAACGTGCATTTTTTATTAATGACCAttTA | |
Nested1 forward Pfmdr1 184 (MDR-A1) | TGTTGAAAGATGGGTAAAGAGCAGAAAGAG | DraI (NEB) |
Nested1 reverse Pfmdr1 184 (MDR-A3) | TACTTTCTTATTACATATGACACCACAAACA | |
Nested2 forward Pfmdr1 184 (MDR-A4) | AAAGATGGTAACCTCAGTATCAAAGAAGAG | |
Nested2 reverse Pfmdr1 184 (MDR-A2) | GTCAAACGTGCATTTTTTATTAATGACCAttTA | |
Nested1 forward Pfmdr1 1246 (MDR-O1) | AGAAGATTATTTCTGTAATTTGATACAAAAAGC | EcoRV (NEB) |
Nested1 reverse Pfmdr1 1246 (MDR-O2) | ATGATTCGATAAATTCATCTATAGCAGCAA | |
Nested2 forward Pfmdr1 1246 (1246F) | ATGATCACATTATATTAAAAAATGATATGACAAAT | |
Nested1 reverse Pfmdr1 1246 (MDR-O2) | ATGATTCGATAAATTCATCTATAGCAGCAA |