Fig. 2
Generation of SGS1 knockout lines. a Number of embryos injected and mutant individuals obtained. b HRMA curve analyses of DNA extracted from legs of G1 male mosquitos. Coloured lines represent mosquitoes with indels confirmed by sequencing. Gray lines represent wild-type LVP mosquitoes. c Gene structure of aaSGS1 along with nucleotide and protein alignment of the knockout lines generated by CRISPR/Cas9. Arrows represent the primer set used for genotyping in HRMA. Wild-type (WT) LVP and mutant SGSΔ25 and SGSΔ13 sequences are indicated. The sgRNA target sequence is underlined; scissors icon indicates the cleavage point with the PAM marked in red. Deleted bases are represented by dashes on the alignment. The red residues are the predicted amino acids generated through the result in frameshift, with (.) indicating a termination codon