Fig. 3

SGS1 protein expression is abrogated in SGS1^ko mosquitoes. a Diagram of workflow. b The number of unique-mapping peptides was linear normalized per 5000 peptides recovered and plotted for both WT and SGS1^ko. Error bars represent the standard deviation based on three biological replicates for both WT (horizontal) and SGS1^ko (vertical). Proteins found to be differentially expressed through EdgeR analysis are shown in red. c Coverage of peptides recovered from proteomic analysis of salivary glands matching to SGS1 protein from wild-type (WT) and SGS1^ko mosquitoes. Red bar indicates the approximate position of the CRISPR/Cas9 target site