Fig. 2

Monoclonal phage ELISA of phage display output clones of round 4 and indirect ELISA of full-size antibodies of phage ELISA positive clones. a 89 output clones of the last phage display panning round (round four) were tested in monoclonal phage ELISA against AMA1 DiCo1-3. Detection was performed using antiM13 antibody. As negative control, providing the background absorbance signal, the supernatant of uninfected XL1-Blue MRF’ was used. b The binding capacity of four selected full-size antibodies was estimated by indirect ELISA using shuffled variants of two heavy and two light chains of two antibodies that were positive for binding against DiCO1-3 in monoclonal phage ELISA. The antibodies were transiently expressed in N. benthamiana. As negative controls, the reaction against PBS and antigen MSP1₁₉ was measured