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Fig. 9 | Malaria Journal

Fig. 9

From: Isolation and light chain shuffling of a Plasmodium falciparum AMA1-specific human monoclonal antibody with growth inhibitory activity

Fig. 9

Pairwise epitope mapping using SPR spectroscopy. For competition measurements of the antibodies generated by phage display among themselves as well as to other described antibodies, the AMA1-antigen DiCo3 was captured on a surface coated with anti-AMA1 antibody 1D7. First the saturating antibody was injected (180 s, 30 µL/min). If there was no binding of the subsequently injected competing antibody, binding competition was assumed. Antibodies were tested against all other available AMA1-antibodies pairwise. Represented are exemplary curves for a non-competing antibody pair (OcheA-17/1E4) (a) and a competing (OcheA-9/OcheA-17) antibody pair (b). The results of all pairwise epitope mappings being performed is summarized (c)

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Malaria Journal

ISSN: 1475-2875

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