Panning round | Washing repeats | Antigen amount (µg/well) | Output/ input phage titer (total enrichment) | |||
---|---|---|---|---|---|---|
pRF-library | pFlx-library | |||||
Helper | Hyper | Helper | Hyper | |||
1 | 10 | 10 | 1.6 × 10–6 | 4.1 × 10–6 | 9.8 × 10–6 | 4.7 × 10–7 |
2 | 25 | 5 | 1.2 × 10–5 (7.5 ×) | 2.3 × 10–6 (0.6 ×) | 1.4 × 10–5 (1.4 ×) | 1.4 × 10–6 (3 ×) |
3 | 40 | 1 | 2.9 × 10–3 (1812.5 ×) | 3.6 × 10–6 (0.9 ×) | 3.5 × 10–4 (35.7 ×) | 8.8 × 10–4 (1872 ×) |