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Table 2 Light chain shuffling results

From: Isolation and light chain shuffling of a Plasmodium falciparum AMA1-specific human monoclonal antibody with growth inhibitory activity

Panning round Washing repeats Antigen amount (µg/well) Output/ input phage titer (total enrichment)
pRF-library pFlx-library
Helper Hyper Helper Hyper
1 10 10 1.6 × 10–6 4.1 × 10–6 9.8 × 10–6 4.7 × 10–7
2 25 5 1.2 × 10–5 (7.5 ×) 2.3 × 10–6 (0.6 ×) 1.4 × 10–5 (1.4 ×) 1.4 × 10–6 (3 ×)
3 40 1 2.9 × 10–3 (1812.5 ×) 3.6 × 10–6 (0.9 ×) 3.5 × 10–4 (35.7 ×) 8.8 × 10–4 (1872 ×)
  1. The output and input titers were titrated for every selection round. The enrichment was calculated by dividing the output/input titers and setting the output/input ratio of round 1 as 1