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Table 2 Light chain shuffling results

From: Isolation and light chain shuffling of a Plasmodium falciparum AMA1-specific human monoclonal antibody with growth inhibitory activity

Panning round

Washing repeats

Antigen amount (µg/well)

Output/ input phage titer (total enrichment)

pRF-library

pFlx-library

Helper

Hyper

Helper

Hyper

1

10

10

1.6 × 10–6

4.1 × 10–6

9.8 × 10–6

4.7 × 10–7

2

25

5

1.2 × 10–5 (7.5 ×)

2.3 × 10–6 (0.6 ×)

1.4 × 10–5 (1.4 ×)

1.4 × 10–6 (3 ×)

3

40

1

2.9 × 10–3 (1812.5 ×)

3.6 × 10–6 (0.9 ×)

3.5 × 10–4 (35.7 ×)

8.8 × 10–4 (1872 ×)

  1. The output and input titers were titrated for every selection round. The enrichment was calculated by dividing the output/input titers and setting the output/input ratio of round 1 as 1