| |
OcheA-9
|
OcheA-16
|
OcheA-17
|
OcheA-17B
|
|---|
|
Expression level (mg/kg FLM)
|
150
|
140
|
440
|
110
|
|
AMA1 DiCo1 KD (nM)
|
–
|
–
|
213
|
43
|
|
AMA1 DiCo2 KD (nM)
|
110
|
–
|
160
|
1750
|
|
AMA1 DiCo3 KD (nM)
|
68
|
55
|
61
|
1.7
|
|
Pf3D7 AMA1 KD (nM)
|
–
|
–
|
–
|
176
|
- To calculate expression levels the concentrations of recombinant antibodies OcheA-9, OcheA-16, OcheA-17 and OcheA-17B in N. benthamiana plant extracts were assessed measuring the capture level on a protein A surface in relation to an internal antibody standard using BiacoreT200. For affinity kinetic measurements purified recombinant antibodies OcheA, OcheA-9, OcheA-16, OcheA-17 and OcheA-17B were captured on a protein A surface (110, 110, 130, 140, 120 RU respectively) and the antigens were injected successively in concentrations of 360 nM, 180 nM and 90 nM for 180 s (30 µL/min). The surface was washed with HBS-EP buffer and regenerated with 30 mM HCl between measurements. Data were evaluated using T200EvaluationSoftware3.0 using the two state reaction binding model. “–”: not determinable
