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Table 1 List of primers used for plasmodium species identification using nested real-time PCR

From: Molecular surveillance and temporal monitoring of malaria parasites in focal Vietnamese provinces

Genus/species

Target

Primer ID

Primer sequence (5′ – 3′)

5′ modified

3′ modified

References

Plasmodium

18S rRNA gene

rPLU6-F

TTAAAATTGTTGCAGTTAAAACG

  

[21]

  

rPLU5-R

CCTGTTGTTGCCTTAAACTTC

   

P. falciparum

18S rRNA type S

PF-F

ATTGCTTTTGAGAGGTTTTGTTACTTT

  

[22]

  

PF-R

GCTGTAGTATTCAAACACAATGAACTCAA

   
  

PF-Probe

CATAACAGACGGGTAGTCAT

HEX

MGBEQ

 

P. vivax

18S rRNA type A

VIV-F

GCAACGCTTCTAGCTTAATCCAC

  

[23]

  

VIV-R

CAAGCCGAAGCAAAGAAAGTCC

   
  

VIV-Probe

ACTTTGTGCGCATTTTGCTA

HEX

MGBEQ

 

P. malariae

18S rRNA gene

PM-F

GGTGTTGGATGATAGAGTAA

  

[20]

  

PM-R

CCCAAAGACTTTGATTTCTC

   
  

PM-Probe

AGGAAGCTATCTAAAAGAAACACTCAT

HEX

BHQ-1

 

P. ovale curtisi

18S rRNA gene

POS-F

ATTTCAAAGAGTCATGGCGTTTCTG

  

[20]

  

POS-R

TTGTAAAGGAGACACTTTCTTGAAATCG

   
  

POS-Probe

CTCCTTGGTCGATCTGCCCAGCACT

FAM

BHQ-1

[20]

P. ovale wallikeri

18S rRNA gene

POS-F

ATTTCAAAGAGTCATGGCGTTTCTG

   
  

POW-R

TGTAAAGGAGACAACTTTCTTGGAGCTA

   
  

POW-Probe

TTGATCGCCCAGCACTGACCATCT

HEX

BHQ-1

 
  1. HEX: 6-hexachlorofluorescein; FAM: 6-carboxyfluorescein; MGBEQ: minor groove binder eclipse quencher. BHQ-1: black hole quencher-1. rRNA: ribosomal ribonucleic acid