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Table 1 Reproducibility of P. falciparum quantification by qPCR

From: Molecular methods for tracking residual Plasmodium falciparum transmission in a close-to-elimination setting in Zanzibar

A

Parasites/µl

Mean (Ct)

SD

%CV

3 µl

15 µl

3 µl

15 µl

3 µl

15 µl

48,800

19.19

18.05

0.15

0.45

0.81

2.49

4880

20.63

19.41

0.06

0.25

0.28

1.26

488

24.34

22.68

0.12

0.24

0.50

1.06

48.8

27.58

25.95

0.12

0.17

0.45

0.64

4.88

31.12

29.76

0.12

0.25

0.39

0.83

2.44

31.91

30.40

0.63

0.49

1.99

1.62

0.48

35.61

32.83

2.01

0.46

5.65

1.39

0.24

34.98

33.78

1.12

0.98

3.20

2.89

B

Parasites/µl

Mean (Ct)

SD

%CV

4880

20.39

0.22

1.07

488

23.72

0.26

1.09

48.8

27.17

0.25

0.93

4.88

30.47

0.38

1.25

0.488

33.35

0.9

2.68

0.048

34.76

1.21

3.49

  1. A. Intra-assay variation of varATS standard curve determined via Ct-values of eight serially diluted DNA samples (in quintuplicate, supplemented with negative DBS) using either 3 µl of DNA (equal to one punch per DBS) or 15 µl of DNA (equal to five punches per DBS)
  2. B. Inter-assay variation of varATS standard curve determined via Ct-values of six serially diluted DNA samples (supplemented by negative DBS) using 3 µl of DNA (equal to one punch per DBS) in 28 replicates