Fig. 1

Global expression profile of mRNAs in the liver of non-vaccinated (N) and vaccination-protected mice (V). a RNA was isolated from individual livers prepared on different days (d0, d1, d4, d8, d11) during infection with P. chabaudi blood-stage malaria. Gene expression levels for the individual livers were determined by microarrays and hierarchically clustered. Log₂ transformed expression levels range from 0 to 18 as indicated at the top with blue (low expression) and red (high expression), respectively. b Hierarchical clustering of liver samples prepared from vaccination-protected (V) and non-vaccinated mice (N) during different phases of infections with P. chabaudi malaria on the indicated days (d0, d1, d4, d8, d11). Suffices indicate the three different livers taken per time point. The correlation metric and the average linkage method were used to construct the dendogram. c Principal Component Analysis (PCA) of gene expression data. The 1st principal component (PC1) captures 40% of the gene expression variability and the 2nd PC2 captures 10% of the variability. The Nd0 populations are depicted by blue spheres, the Nd1 by cyan spheres, the Nd4 by green spheres, the Nd8 by magenta spheres, the Nd11 by red spheres, the Vd0 by blue icosahedra, the Vd1 by cyan icosahedra, the Vd4 by green icosahedra, the Vd8 by magenta icosahedra and the Vd11 by red icosahedra