Subject 1 | Subject 2 | Subject 3 | Subject 4# | |
---|---|---|---|---|
Apheresis procedure | CMNC | CMNC | CMNC | Red cell depletion followed by CMNC on red cell depletion product |
HCT layers sampled* | 1%, 2%, 3%, 5%, 7% | 1%, 2%, 3%, 5%, 7% | 0.5%, 1%, 2%, 3%, 5%, 7%, 11%+ 2–3%, 5–7%, 1–7% | From the primary apheresis (HCT): Intermediate (64%) From the secondary apheresis (HCT): Final (3%) Spare (5%) Waste (42%) |
Apheresis timepoint | 10 | 10 | 11 PM | 11 AM |
Mosquito feeding assay samples | Pre-apheresis (with-Percoll enrichment), 1%, 2%, 3% HCT layers | Pre-apheresis (with-Percoll enrichment) | Pre-apheresis (with and without-Percoll enrichment) | Pre-apheresis (without-Percoll enrichment), Intermediate, Final, Waste |
Whole blood:citrate ratio during apheresis | 15:1 | 8:1 | 8:1 | 13:1 |
Citrate added to apheresis collection bags | No | Yes | Yes | Yes |
Biological duplicates* | No | No | Yes | Yes |