Safety and feasibility of apheresis to harvest and concentrate parasites from subjects with induced blood stage Plasmodium vivax infection

Table 3 Mosquito infection rates following membrane feeding assays

Sample	Subject 1				Subject 2	Subject 3		Subject 4
	Pre-apheresis (with-Percoll enrichment)	Apheresis samples			Pre-apheresis (with-Percoll enrichment)	Pre-apheresis (without-Percoll enrichment)	Pre-apheresis (with-Percoll enrichment)	^aPre-apheresis (without-Percoll enrichment)	Apheresis samples
	Pre-apheresis (with-Percoll enrichment)	1% HCT	2% HCT	3% HCT	Pre-apheresis (with-Percoll enrichment)	Pre-apheresis (without-Percoll enrichment)	Pre-apheresis (with-Percoll enrichment)	^aPre-apheresis (without-Percoll enrichment)	Intermediate (64% HCT)	Final (3% HCT)	Waste (42% HCT)
Feeding rate (No. mosquitos fed/No. total mosquitos [%])	127/133 [95.5%]	101/103 [98.1%]	103/104 [99%]	114/116 [98.3%]	75/77 [97.4%]	102/103 [99%]	107/107 [100%]	29/29 [100%]	28/29 [97%]	26/26 [100%]	30/30 [100%]
Mortality rate (No. dead mosquitos/No. total mosquitos [%])	23/133 [17.3%]	3/103 [2.9%]	6/104 [5.8%]	11/116 [9.5%]	7/77 [9.1%]	6/103 [5.8%]	5/107 [4.7%]	1/29 [3.4%]	3/29 [10.3%]	1/26 [3.8%]	1/30 [3.3%]
Infection rate (No. mosquitoes with oocysts/No. mosquitos tested [%])	0/110[0%]	0/105 [0%]	0/98 [0%]	0/105 [0%]	0/77 [0%]	16/93 [17.2%]	99/100 [99%]	1/28 [3.6%]	1/25 [4%]	5/25 [20%]	0/24 [0%]

Only subjects 1 and 4 involved the testing of samples collected using apheresis. For logistic reasons, MFA was not carried out in subject 4. Following consideration of gametocyte levels, DMFA was not carried out in subject 2. Reported parameters include feeding rate of mosquitoes, adult mosquito mortality rate after feeding and mosquito infection rate
^aMFA without Percoll enrichment to save on blood volume draw for safety reasons

ISSN: 1475-2875