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Fig. 3 | Malaria Journal

Fig. 3

From: Multiplex-RT-PCR-ELISA panel for detecting mosquito-borne pathogens: Plasmodium sp. preserved and eluted from dried blood spots on sample cards

Fig. 3

Differentiation of Plasmodium sp. and DENV serotypes. Plasmodium falciparum, P. vivax and P. malariae in species-positive sera derived from infected patients were amplified by multiplex-RT-PCR and then differentiated by 2% agarose gel electrophoresis (a). Inactivated DENV particles were amplified and serotyped by singleplex PCR (b). MW molecular weight marker pUC19 DNA/MspI and the following species or serotypes: P. falciparum, 100 bp; P. vivax, 141 bp; P. malariae, 166 bp; DENV-1, 208 bp; DENV-2, 119 bp; DENV-3, 288 bp; and DENV-4, 260 bp; Influenza A, 190 bp served as a positive control; NaCl and nuclease-free H2O served as negative isolation (NeCo Iso) and primer mix controls (NeCo PCR), respectively

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