Table 3 Multiplex-RT-PCR-ELISA positive controls
| Â | Family | No | Pathogen | Source | Positive control |
|---|---|---|---|---|---|
Arbo viruses | Flaviviridae | 1 | Dengue virus (DENV) | Laboratory of Pediatric Immunology and Infectious Diseases, Mainz, Germany | Inactivated particles: DENV serotypes 1, 2, 3 and 4 |
2 | West Nile virus (WNV) | HiSS Diagnostics GmbH, Freiburg im Breisgau, Germany | Inactivated cell culture supernatant: Accu Type TM West Nile virus lineage I | ||
3 | Zika virus (ZIKV) | Robert Koch Institute, Berlin, Germany | Inactivated cell culture supernatant (strain MR766-infected Vero E6 cells); aliquoted and freeze-dried | ||
Togaviridae (Alphaviruses) | 4 | Yellow Fever virus (YFV) | Robert Koch Institute, Berlin, Germany | Inactivated cell culture supernatant | |
| Â | 5 | Semliki Forest virus (SFV) | European Virus Archive goes Global, Marseille, France | Original isolate. RNA prepared from cell culture supernatant using QIAamp Viral RNA Mini Kit, Qiagen | |
6 | O´nyong-nyong virus (ONNV) | European Virus Archive goes Global | Inactivated cell culture supernatant (strain Dakar 234-infected Vero cells); freeze-dried sample | ||
7 | Chikungunya virus (CHIKV) | Institut for Virology- Universitätsklinikum, Freiburg, Germany | Inactivated virus particles | ||
Bunyaviridae | 8 | Rift Valley Fever virus (RVFV) | Robert Koch Institute, Berlin, Germany | Inactivated cell culture supernatant | |
Protozoa | Malaria (Plasmodium sp.) | 9 | P. falciparum P. vivax P. malariae | Discovery Life Sciences, Los Osos, CA, USA | Serum obtained from patients with acute malaria infection (P. falciparum, P. vivax, and P. malariae). Microscopy results: parasite-positive |