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Table 3 Multiplex-RT-PCR-ELISA positive controls

From: Multiplex-RT-PCR-ELISA panel for detecting mosquito-borne pathogens: Plasmodium sp. preserved and eluted from dried blood spots on sample cards

  Family No Pathogen Source Positive control
Arbo viruses Flaviviridae 1 Dengue virus (DENV) Laboratory of Pediatric Immunology and Infectious Diseases, Mainz, Germany Inactivated particles: DENV serotypes 1, 2, 3 and 4
2 West Nile virus (WNV) HiSS Diagnostics GmbH, Freiburg im Breisgau, Germany Inactivated cell culture supernatant: Accu Type TM West Nile virus lineage I
3 Zika virus (ZIKV) Robert Koch Institute, Berlin, Germany Inactivated cell culture supernatant (strain MR766-infected Vero E6 cells); aliquoted and freeze-dried
Togaviridae (Alphaviruses) 4 Yellow Fever virus (YFV) Robert Koch Institute, Berlin, Germany Inactivated cell culture supernatant
  5 Semliki Forest virus (SFV) European Virus Archive goes Global, Marseille, France Original isolate. RNA prepared from cell culture supernatant using QIAamp Viral RNA Mini Kit, Qiagen
6 O´nyong-nyong virus (ONNV) European Virus Archive goes Global Inactivated cell culture supernatant (strain Dakar 234-infected Vero cells); freeze-dried sample
7 Chikungunya virus (CHIKV) Institut for Virology- Universitätsklinikum, Freiburg, Germany Inactivated virus particles
Bunyaviridae 8 Rift Valley Fever virus (RVFV) Robert Koch Institute, Berlin, Germany Inactivated cell culture supernatant
Protozoa Malaria (Plasmodium sp.) 9 P. falciparum
P. vivax
P. malariae
Discovery Life Sciences, Los Osos, CA, USA Serum obtained from patients with acute malaria infection (P. falciparum, P. vivax, and P. malariae). Microscopy results: parasite-positive
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