Virus RNA copies/ml
|
1 × 106
|
1 × 105
|
1 × 104
|
1 × 103
|
1 × 102
|
1 × 101
|
1 × 100
|
---|
ELISAb (OD405)
|
1.45 ± 0.05
|
1.55 ± 0.19
|
1.06 ± 0.17
|
0.41 ± 0.11
|
0.17 ± 0.13
|
0.09 ± 0.01
|
0.09 ± 0.03
|
- aThe MR766 strain of ZIKV (1 × 107 viral RNA copies/ml) was serially diluted 1:10 and multiplex-RT-PCR-ELISA was conducted to determine the limits of detection
- bValues are the means ± SEM calculated from 3 determinations. OD readings ≥ 4-times the negative control (0.1 OD405) were judged positive [27]. Values 2- to 4-times the negative control were considered borderline