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Table 5 Multiplex-RT-PCR-ELISA detection limit Zika-Virusa

From: Multiplex-RT-PCR-ELISA panel for detecting mosquito-borne pathogens: Plasmodium sp. preserved and eluted from dried blood spots on sample cards

Virus RNA copies/ml

1 × 106

1 × 105

1 × 104

1 × 103

1 × 102

1 × 101

1 × 100

ELISAb (OD405)

1.45 ± 0.05

1.55 ± 0.19

1.06 ± 0.17

0.41 ± 0.11

0.17 ± 0.13

0.09 ± 0.01

0.09 ± 0.03

  1. aThe MR766 strain of ZIKV (1 × 107 viral RNA copies/ml) was serially diluted 1:10 and multiplex-RT-PCR-ELISA was conducted to determine the limits of detection
  2. bValues are the means ± SEM calculated from 3 determinations. OD readings ≥ 4-times the negative control (0.1 OD405) were judged positive [27]. Values 2- to 4-times the negative control were considered borderline
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Malaria Journal

ISSN: 1475-2875

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