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Table 7 Efficacy of multiplex-RT-PCR-ELISA conducted on positive controls eluted from sample cards

From: Multiplex-RT-PCR-ELISA panel for detecting mosquito-borne pathogens: Plasmodium sp. preserved and eluted from dried blood spots on sample cards

Pathogensa P. fal DENV ZIKA RVF CHIKV WNV YFV
Elution Klüber method
 ELISA (OD405)
0.77 ± 0.1 0.59 ± 0.26 0.42 ± 0.25 1.49 ± 0.13 0.59 ± 0.24 0.38 ± 0.27 1.32 ± 0.54
Elution Klüber-Gruner method
 ELISA (OD405)
1.17 ± 0.25 0.41 ± 0.04 0.26 ± 0.07 1.44 ± 0.42 0.33 ± 0.07 0.33 ± 0.1 0.44 ± 0.23
Elution Gruner method
 ELISA (OD405)
0.6 ± 0.14 0.3 ± 0.02 0.25 ± 0.02 0.8 ± 0.1 0.24 ± 0.04 0.23 ± 0.04 0.47 ± 0.24
Not spotted
 ELISA (OD405)
2.31 ± 0.01 1.65 ± 0.07 2.12 ± 0.05 1.72 ± 0.09 1.27 ± 0.04 1.67 ± 0.11 2.04 ± 0.08
  1. aData, normalized based upon the OD405 values generated from pooled influenza A amplicons, are the means ± SEM obtained from triplicate plates shown in Fig. 3. OD readings ≥ 4-times the negative control (0.1 OD405) were judged to be positive
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