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Table 7 Efficacy of multiplex-RT-PCR-ELISA conducted on positive controls eluted from sample cards

From: Multiplex-RT-PCR-ELISA panel for detecting mosquito-borne pathogens: Plasmodium sp. preserved and eluted from dried blood spots on sample cards

Pathogensa

P. fal

DENV

ZIKA

RVF

CHIKV

WNV

YFV

Elution Klüber method

 ELISA (OD405)

0.77 ± 0.1

0.59 ± 0.26

0.42 ± 0.25

1.49 ± 0.13

0.59 ± 0.24

0.38 ± 0.27

1.32 ± 0.54

Elution Klüber-Gruner method

 ELISA (OD405)

1.17 ± 0.25

0.41 ± 0.04

0.26 ± 0.07

1.44 ± 0.42

0.33 ± 0.07

0.33 ± 0.1

0.44 ± 0.23

Elution Gruner method

 ELISA (OD405)

0.6 ± 0.14

0.3 ± 0.02

0.25 ± 0.02

0.8 ± 0.1

0.24 ± 0.04

0.23 ± 0.04

0.47 ± 0.24

Not spotted

 ELISA (OD405)

2.31 ± 0.01

1.65 ± 0.07

2.12 ± 0.05

1.72 ± 0.09

1.27 ± 0.04

1.67 ± 0.11

2.04 ± 0.08

  1. aData, normalized based upon the OD405 values generated from pooled influenza A amplicons, are the means ± SEM obtained from triplicate plates shown in Fig. 3. OD readings ≥ 4-times the negative control (0.1 OD405) were judged to be positive