Multiplex-RT-PCR-ELISA panel for detecting mosquito-borne pathogens: Plasmodium sp. preserved and eluted from dried blood spots on sample cards

Table 9 Efficacy of standard diagnostic tools for detecting Plasmodium sp.

	Sensitivity (+/+)	Specificity (−/−)
Blood smear microscopy, n = 122	40.0% (10/25)	92.8% (90/97)
mRDT (NADAL^®), n = 132	96.3% (26/27)	98.1% (103/105)

The presence of Plasmodium sp. in blood samples obtained from 132 children suspected of having malaria was assessed by blood smear microscopy and mRDT. The results were compared to those obtained for the same patient cohort that tested positive by multiplex-RT-PCR-ELISA, i.e., 27 of 132

ISSN: 1475-2875