From: High frequency of the Duffy-negative genotype and absence of Plasmodium vivax infections in Ghana
Purpose [Ref.] | Primer name | Sequence (5′–3′) | Amplicon (bp) | PCR conditions |
---|---|---|---|---|
Plasmodium sp. [32] | rPLU5 | CCTGTTGTTGCCTTAAACTTC | 1100 | 94 °C × 5 min, 35 cycles (94 °C × 60 s, 60 °C × 90 s, 68 °C × 60 s), 68 °C × 10 min |
rPLU6 | TTAAAATTGTTGCAGTTAAAAC | |||
P. falciparum [32] | rFAL1 | TTAAACTGGTTTGGGAAAACCAAATATATT | 205 | 94 °C × 5 min, 35 cycles (94 °C × 60 s, 55 °C × 90 s, 68 °C × 60 s), 68 °C × 10 min |
rFAL2 | ACACAATGAACTCAATCATGACTACCCGTC | |||
P. vivax [32] | rVIV1 | CGCTTCTAGCTTAATCCACATAACTGATAC | 120 | 94 °C × 5 min, 35 cycles (94 °C × 60 s, 55 °C × 90 s, 68 °C × 60 s), 68 °C × 10 min |
rVIV2 | ACTTCCAAGCCGAAGCAAAGAAAGTCCTTA | |||
P. malariae [32] | rMAL1 | ATAACATAGTTGTACGTTAAGAATAACCGC | 144 | 94 °C × 5 min, 35 cycles (94 °C × 60 s, 55 °C × 90 s, 68 °C × 60 s), 68 °C × 10 min |
rMAL2 | AAAATTCCCATGCATAAAAAATTATACAAA | |||
P. ovale [32] | rOVA1 | ATCTCTTTTGCTATTTTTTAGTATTGGAGA | 800 | 94 °C × 5 min, 35 cycles (94 °C × 60 s, 55 °C × 90 s, 68 °C × 60 s), 68 °C × 10 min |
rOVA2 | GAAAAGGACACATTAATTGTATCCTAGTG | |||
P. vivax [33] | VivF | TCCATCCTGTTGGTGGACTT | 700 | 94 °C × 5 min, 35 cycles (94 °C × 60 s, 60 °C × 90 s, 68 °C × 60 s), 68 °C × 10 min |
VivR | TCACAACGTTAAATATGCCAG | |||
Duffy genotypes [34] | GATAFY2 | CTCATTAGTCCTTGGCTCTTAC | 711 | 94 °C for 5 min, 40 cycles (94 °C × 30 s, 56 °C × 30 s, 68 °C × 60 s), 68 °C × 10 min |
FYAB2 | CTCATTAGTCCTTGGCTCTTAT | |||
FYAREV | AGCTGCTTCCAGGTTGGCAC | |||
FYBREV | AGCTGCTTCCAGGTTGGCAT | |||
Human growth hormone gene [35] | HGH-F | TGCCTTCCCAACCATTCCCTTA | 434 | 94 °C for 5 min, 40 cycles (94 °C × 30 s, 56 °C × 30 s, 68 °C × 60 s), 68 °C × 10 min |
HGH-R | CCACTCACGGATTTCTGTTGTGTTTC |