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Table 1 Oligonucleotide primers and PCR conditions used in this study

From: High frequency of the Duffy-negative genotype and absence of Plasmodium vivax infections in Ghana

Purpose [Ref.]

Primer name

Sequence (5′–3′)

Amplicon (bp)

PCR conditions

Plasmodium sp. [32]

rPLU5

CCTGTTGTTGCCTTAAACTTC

1100

94 °C × 5 min, 35 cycles (94 °C × 60 s, 60 °C × 90 s, 68 °C × 60 s), 68 °C × 10 min

rPLU6

TTAAAATTGTTGCAGTTAAAAC

P. falciparum [32]

rFAL1

TTAAACTGGTTTGGGAAAACCAAATATATT

205

94 °C × 5 min, 35 cycles (94 °C × 60 s, 55 °C × 90 s, 68 °C × 60 s), 68 °C × 10 min

rFAL2

ACACAATGAACTCAATCATGACTACCCGTC

P. vivax [32]

rVIV1

CGCTTCTAGCTTAATCCACATAACTGATAC

120

94 °C × 5 min, 35 cycles (94 °C × 60 s, 55 °C × 90 s, 68 °C × 60 s), 68 °C × 10 min

rVIV2

ACTTCCAAGCCGAAGCAAAGAAAGTCCTTA

P. malariae [32]

rMAL1

ATAACATAGTTGTACGTTAAGAATAACCGC

144

94 °C × 5 min, 35 cycles (94 °C × 60 s, 55 °C × 90 s, 68 °C × 60 s), 68 °C × 10 min

rMAL2

AAAATTCCCATGCATAAAAAATTATACAAA

P. ovale [32]

rOVA1

ATCTCTTTTGCTATTTTTTAGTATTGGAGA

800

94 °C × 5 min, 35 cycles (94 °C × 60 s, 55 °C × 90 s, 68 °C × 60 s), 68 °C × 10 min

rOVA2

GAAAAGGACACATTAATTGTATCCTAGTG

P. vivax [33]

VivF

TCCATCCTGTTGGTGGACTT

700

94 °C × 5 min, 35 cycles (94 °C × 60 s, 60 °C × 90 s, 68 °C × 60 s), 68 °C × 10 min

VivR

TCACAACGTTAAATATGCCAG

Duffy genotypes [34]

GATAFY2

CTCATTAGTCCTTGGCTCTTAC

711

94 °C for 5 min, 40 cycles (94 °C × 30 s, 56 °C × 30 s, 68 °C × 60 s), 68 °C × 10 min

FYAB2

CTCATTAGTCCTTGGCTCTTAT

FYAREV

AGCTGCTTCCAGGTTGGCAC

FYBREV

AGCTGCTTCCAGGTTGGCAT

Human growth hormone gene [35]

HGH-F

TGCCTTCCCAACCATTCCCTTA

434

94 °C for 5 min, 40 cycles (94 °C × 30 s, 56 °C × 30 s, 68 °C × 60 s), 68 °C × 10 min

HGH-R

CCACTCACGGATTTCTGTTGTGTTTC