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Fig. 5 | Malaria Journal

Fig. 5

From: Development of a new barcode-based, multiplex-PCR, next-generation-sequencing assay and data processing and analytical pipeline for multiplicity of infection detection of Plasmodium falciparum

Fig. 5

Metrics for assessing the change in transmission level in western Kenya. a EIR in Asembo, western Kenya from 1995 to 2012. b Malaria prevalence in age under 5 years old with a 95% confidence interval in same area from 1996 to 2012. c MOI as estimated by the STIM algorithm from 1996, 2001, 2007, and 2012 surveys in same area, presented as average MOI. d Percentage of samples with different number of strains in same area from 1996, 2001, 2007, and 2012 surveys. EIR data and malaria prevalence data, based on smear diagnosis, were extracted from published [38] and unpublished (KEMRI/CDC) data. The StrainRecon and STIM algorithms with a threshold of \(T = 1.8 \times 10^{ - 7}\) were run for the SNP frequency data generated from smear-positive samples from 4 surveys in Kenya for MOI estimation

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