Fig. 6From: Development of a new barcode-based, multiplex-PCR, next-generation-sequencing assay and data processing and analytical pipeline for multiplicity of infection detection of Plasmodium falciparumFDR-adjusted significance levels of the key statistical tests with different STIM thresholds T. Robustness across a neighborhood of values around the chosen T, shown on a log–log scale. The dotted black vertical line is the threshold derived from laboratory data and was used to generate Fig. 5c, d and Table 3; the dotted blue horizontal line is the significance level of \(q = 0.05\). The strain count was not significantly different between 2007 and 2012 in the vicinity of the MOI misfit threshold usedBack to article page