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Table 1 Barcode reconstruction quality with StrainRecon, varying the number of strains to be found

From: Development of a new barcode-based, multiplex-PCR, next-generation-sequencing assay and data processing and analytical pipeline for multiplicity of infection detection of Plasmodium falciparum

Proportion of SNP sites correctly reconstructed Strain 1 (%) Strain 2 (%) Strain 3 (%) Strain 4a (%)
Anticipated MOI n = 1 98.97    
Anticipated MOI n = 2 98.97 84.79   
Anticipated MOI n = 3 98.70 89.25 64.64  
Anticipated MOI n = 4 98.78 82.69 48.80 19.79
Anticipated MOI n = 5 98.50 77.52 47.06 19.28
  1. Columns are arranged by proportion of strain in the mixture, so the most dominant strain is called Strain 1. Non-barcode SNP sites are omitted
  2. aStrain 4 exists only in those samples where SNP23 had a mutation
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