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Fig. 2 | Malaria Journal

Fig. 2

From: Natural infections with different Plasmodium species induce antibodies reactive to a chimeric Plasmodium vivax recombinant protein

Fig. 2

Capture of rabbit and murine anti-PvRMC-MSP1 antibodies with MSP1 proteins from the four Plasmodium species. a IgG assay signal for purified total IgG obtained from rabbits following four immunizations with the PvRMC-MSP1 protein. Median fluorescence intensity minus background (MFI-bg) assay signal is shown for each of the four MSP1 proteins derived from human Plasmodium species in comparison to the PvRMC-MSP1 used for immunization. b IgG assay signal for mouse sera obtained after three immunizations with PvRMC-MSP1 with MSP1 proteins from the four human Plasmodium species. c Plasmodium falciparum NF54-infected red blood cells smeared on glass slides were incubated with sera obtained from immunized rabbits (top), and plasma from individuals living in malaria-endemic regions (bottom). Panels show staining for DNA (DAPI, blue), IgG (AlexaFluor 488, green), and merge. All images are shown at 100 × magnification, and the scale bar indicates 5 µm

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