Development and evaluation of a new Plasmodium falciparum 3D7 blood stage malaria cell bank for use in malaria volunteer infection studies

Table 1 Summary of parasite growth and clearance characteristics

Parameter	3D7-V1 (n = 2) Estimate (95% CI)	3D7-MBE-008 (n = 2) Estimate (95% CI)	3D7-V1 1995 (n = 1) Estimate (95% CI)	3D7-V2 (n = 177) Estimate (95% CI) [25]
PMR₄₈	11.5 (8.5–15.6)	34.6 (18.5–64.6)	6.4 (4.6–8.8)	31.9 (28.7–35.4)
Lifecycle (hours)	36.5 (35.0–38.0)	38.8 (36.7–40.8)	42.5 (39.2–45.8)	38.8 (38.3–39.2)
Growth rate (log₁₀ parasites/day)	0.52 (0.30–0.74)	0.80 (0.54 –1.10)	0.35 (0.13–0.58)	0.63 (0.59–0.66)
PRR₄₈	2254 (1705–2979)	3806 (1446–10018)	_	_
log10 PRR₄₈	3.35 (3.23–3.47)	3.58 (3.16–4.00)	_	_
Parasite clearance half life	4.31 (4.16–4.47)	4.04 (3.61–4.57)	_	_

n, number of participants; CI, 95% confidence intervals; PMR, parasite multiplication rate; PRR, parasite reduction ratio. Growth parameter estimates are calculated from a non-linear mixed effects model. Clearance parameter estimates (PRR₄₈, log₁₀ PRR₄₈ and parasite clearance half-life) are calculated from a weighted mean of individual clearance parameters estimates

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