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Table 4 Parasite densities and PfHRP2 RDT results among PCR double deletions of pfhrp2 exon2 and pfhrp3 exon2 genes

From: Detection of high prevalence of Plasmodium falciparum histidine-rich protein 2/3 gene deletions in Assosa zone, Ethiopia: implication for malaria diagnosis

Sample ID

Parasite density/µl as determined by microscopy

Parasite density/µl as determined by qPCR

PfHRP2 RDT results

Pfhrp2 gene including the flanking regions

Phrp3 gene including the flanking region

MAL7P1_230

Pfhrp2 Exon1-2

Pfhrp2 Exon2

MAL7P1_228

MAL13P1_475

Pfhrp3 Exon1-2

Pfhrp3 exon2

MAL13P1_485

HShr154

4800

1406.49

−

−

−

−

−

−

−

−

−

Hkum68

__

23,827,545

−

−

−

−

−

−

 + 

−

−

LShr106

__

134.61

−

−

−

−

−

−

−

−

−

LBa16

__

999.1

−

−

−

−

−

−

 + 

−

−

Lkum59

10,000

773.06

−

−

−

−

−

−

 + 

−

−

  1.  +  = Positive,,− = Negative,. Upstream (PF3D7_0831900(MAL7P1_230) and downstream (PF3D7_0831700(MAL7P1_228) flanking regions of Pfhrp2. Upstream (PF3D7_1372100(MAL13P1_475)) and) downstream (PF3D7_1372400(MAL13P1_485)) flanking regions of pfhrp3