Genetic characterization of Plasmodium vivax isolates from Pakistan using circumsporozoite protein (pvcsp) and merozoite surface protein-1 (pvmsp-1) genes as genetic markers

Table 2 Estimates of nucleotide, haplotype-diversity and DNA sequence polymorphisms of CRR region of P. vivax pvcsp and block 2 of pvmsp-1 genes in Pakistan

Population	Gene analysed	No. of sequences	Fragment studied	Size of the fragment	No. of Haplotypes	Diversity ± SD	Fu & Li’s D*	Fu & Li’s F*	Tajima’s D*
Population	Gene analysed	No. of sequences	Fragment studied	(codons)#	No. of Haplotypes	Nucleotide (π) Haplotype	Fu & Li’s D*	Fu & Li’s F*	Tajima’s D*
Pakistan	pvcsp	35	CRR	364	15	0.02371 ± 0.00056^† 0.084 ± 0.00701^†	1.1787	1.12083	0.54276
Iran	pvcsp	28	CRR	360	10	0.02001 ± 0.00031^† 0.057 ± 0.00478^†	1.0198	1.80767	0.43556
Myanmar	pvcsp	15	CRR	354	7	0.01781 ± 0.0008† 0.056 ± 0.015	− 1.20965	− 1.06781	− 0.78645
India	pvcsp	25	CRR	324	17	0.0370 ± 0.0064† 0.681 ± 0.076	1.05422	1.02237	0.35674
Pakistan	pvmsp-1	30	block 2	143	10	0.00162 ± 0.0000026^† 0.012 ± 0.00014^†	1.86276	2.13897	1.6779
Iran	pvmsp-1	32	block 2	151	10	0.00159 ± 0.0000023^† 0.012 ± 0.00014^†	1.78902	1.90878	1.56845
India	pvmsp-1	25	block 2	155	7	0.0212 ± 0.0005† 0.989 ± 0.010	1.55433	1.744009	1.66792

ISSN: 1475-2875