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Fig. 2 | Malaria Journal

Fig. 2

From: Surveillance of Plasmodium falciparum pfcrt haplotypes in southwestern Uganda by high‐resolution melt analysis

Fig. 2

Summary of quantitative PCR and High-Resolution Melt parameters and analysis. Representative graphs of quantitative PCR and High-Resolution Melt analysis of clinical samples (thin coloured lines) and control samples (bold lines, blue: CVMNK wild type, HB3 parasite line; red: mutant SVMNT haplotype, 7G8 parasite line; purple: mutant CVIET haplotype, 7C424 parasite line). All analysis was performed with Rotor-Gene Q software and colours and line thickness were modified with TeeChart. a LCGreen fluorescence-based real time detection of DNA amplification of clinical samples and genomic DNA controls. Horizontal red line denotes the Ct threshold, which was determined for each assay. b A derivative plot displaying the melt analysis curve highlighting probe dissociation between normalization regions (denoted by dashed lines at 51 oC and 71 oC). The change in fluorescence was recorded in 0.2 oC increments every 2 s. c Difference curve normalized to the no template control (NTC). The shape of the curve between the normalization region (see panel B) dictates the automatic genotype call

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