Fig. 3

High-Resolution Melt analysis results from various sample sources and PCR-HRM mixes. Ct values (from quantitative PCR) and confidence percentages (from High-Resolution Melt analysis haplotype calls) display comparable results between Group 1 (a) and Group 2 (b) samples despite variable sources and PCR-HRM master mixes. Confidence percentages are automatically assigned by Rotor-Gene Q software to each sample according to their HRM melt profiles. Manual calls were set to a confidence percentage of 100 %. Samples with Ct values of > 38 cycles were excluded from the analysis (indicated as grey triangle).-G, Giemsa-stained blood slides; -B, blood pellets; CVMNK, wild type; SVMNT, mutant; CVIET, mutant