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Fig. 4 | Malaria Journal

Fig. 4

From: Surveillance of Plasmodium falciparum pfcrt haplotypes in southwestern Uganda by high‐resolution melt analysis

Fig. 4

High-Resolution Melt analysis of mixed pfcrt haplotypes using the Rotor-Gene Q instrument. Derivative plots displaying the melt analysis curve for a 50:50 haplotype mixture of reference genomic DNA (a, SVMNT:CVIET), 30:30:30 mixture (b, CVMNK:SVMNT:CVIET), and 90:10 mixture (c, SVMNT:CVMNK). The heteroduplex ‘shoulder’ (arrow) is due to the formation and melting of a heteroduplex of both full length amplicon sequences. d Summary table of genotype, confidence % called by Rotor-Gene Q, and corresponding line colour

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Malaria Journal

ISSN: 1475-2875

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