Surveillance of Plasmodium falciparum pfcrt haplotypes in southwestern Uganda by high‐resolution melt analysis

Malaria Journal

Table 1 Summary of High-Resolution Melt assay results and important metrics

Group	No. samples^a	pfcrt haplotype		No. called (%)	Mean confidence %^d	Mean Ct Value
1^b	119	CVMNK	Wild type	6 (5.0) MC: 2	84.6	30.2 MC: 34.0
		SVMNT	Mutant	0 (0 %)	N/A	N/A
		CVIET	Mutant	112 (94.1) MC:11	93.8	28.1 MC: 34.4
		Unknown	Variation	1 (0.8)	n.d.	30.1
					Overall Mean: 93.5 %	Overall Mean: 28.2
2^c	114	CVMNK	Wild type	60 (52.6)	88.2	31.6
		SVMNT	Mutant	1 (0.9 %)	33.6^e	*26.8
		CVIET	Mutant	50 (43.9 %)	91.5	30.2
		Unknown	Variation	3 (2.6 %)	n.d.	35.1
					Overall Mean: 89.7 %	Overall Mean: 31.1

^aAnalyzed samples only include those with a Ct value < 38
^bDNA samples were purified from predominantly Giemsa-stained slides
^cDNA samples were purified from blood spots on filter paper
^dMean confidence % is assigned by software when automatic calls were made
^eDenotes the exact value since an average could not be calculated; omitted from total average calculation
Underline denotes amino acid changes from the wild type CVMNK haplotype
MC Manual Calls, CVMNK Cys-Val-Met-Asn-Lys, SVMNT Ser-Val-Met-Asn-Thr, CVIET Cys-Val-Ile-Glu-Thr, N/A not applicable, n.d. not determined (a confidence value could not be assigned for these clinical samples due to their unknown melting profile)

ISSN: 1475-2875