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Table 1 Summary of High-Resolution Melt assay results and important metrics

From: Surveillance of Plasmodium falciparum pfcrt haplotypes in southwestern Uganda by high‐resolution melt analysis

Group No. samplesa pfcrt haplotype No. called (%) Mean confidence %d Mean Ct
Value
1b 119 CVMNK Wild type 6 (5.0)
MC: 2
84.6 30.2
MC: 34.0
SVMNT Mutant 0 (0 %) N/A N/A
CVIET Mutant 112 (94.1)
MC:11
93.8 28.1
MC: 34.4
Unknown Variation 1 (0.8) n.d. 30.1
    Overall Mean: 93.5 % Overall Mean: 28.2
2c 114 CVMNK Wild type 60 (52.6) 88.2 31.6
SVMNT Mutant 1 (0.9 %) 33.6e *26.8
CVIET Mutant 50 (43.9 %) 91.5 30.2
Unknown Variation 3 (2.6 %) n.d. 35.1
    Overall Mean: 89.7 % Overall Mean: 31.1
  1. aAnalyzed samples only include those with a Ct value < 38
  2. bDNA samples were purified from predominantly Giemsa-stained slides
  3. cDNA samples were purified from blood spots on filter paper
  4. dMean confidence % is assigned by software when automatic calls were made
  5. eDenotes the exact value since an average could not be calculated; omitted from total average calculation
  6. Underline denotes amino acid changes from the wild type CVMNK haplotype
  7. MC Manual Calls, CVMNK Cys-Val-Met-Asn-Lys, SVMNT Ser-Val-Met-Asn-Thr, CVIET Cys-Val-Ile-Glu-Thr, N/A not applicable, n.d. not determined (a confidence value could not be assigned for these clinical samples due to their unknown melting profile)