Measurement of gene amplifications related to drug resistance in Plasmodium falciparum using droplet digital PCR

Table 1 Descriptive statistics of P. falciparum mdr1, plasmepsin2, and gch1 CNVs based on the accepted lamda(λ) value of ddPCR assay of pf-β-tubulin gene

Statistic	pfmdr1 CNVs			pfplasmepsin2 CNVs			pfgch1 CNVs
Statistic	Singleplex	Duplex	Multiplex	Singleplex	Duplex	Multiplex	Singleplex	Duplex
Range	0.253	0.177	0.225	0.315	0.276	0.214	0.324	0.236
Minimum	0.812	0.817	0.861	0.814	0.738	0.813	0.868	0.926
Maximum	1.065	0.994	1.086	1.129	1.014	1.027	1.192	1.162
Mean	0.922	0.919	0.964	0.943	0.895	0.906	1.016	1.039
SD	0.061	0.040	0.061	0.085	0.057	0.057	0.078	0.065
Variance	0.004	0.002	0.004	0.007	0.003	0.003	0.006	0.004
%RSD	6.954	3.273	4.755	8.675	5.947	5.385	5.282	3.705
%Accuracy	92.240	92.135	95.047	93.304	89.533	90.638	95.118	94.876
Accepted lamda(λ) range	0.011–0.976	0.005–2.173	0.003–0.839	0.010–1.860	0.002–1.890	0.003–0.938	0.006–1.909	0.003–1.874

ISSN: 1475-2875